具体实施方式 DETAILED DESCRIPTION

[0021] 为了防治农业生产上的蚜虫，发明人以环氧虫啉分别与联苯菊酯、高效氯氟氰菊酯进行了相互复配的增效研宄。 [0021] In order to control aphids on agricultural production, the inventors epoxy insect morpholine respectively bifenthrin, cyhalothrin were mutually synergistic complex research traitor. 试验采用苹果黄蚜为测试对象，具体方法为： Test uses aphid test object, specific methods are:

[0022] 苹果黄财（Aphis pomi DeGeer)，又名绣线菊财，属同翅目，财科。 [0022] Apple Huang Choi (Aphis pomi DeGeer), also known as Spiraea fiscal, Homoptera, Finance Division. 将原药配制成需要的试验药剂，试验方法参考《中华人民共和国农业行业标准NY/T1154. 6-2006》。 Original drug formulated to require drug tests, test methods refer to "People's Republic of China agricultural industry standards NY / T1154. 6-2006". 首先将单剂及各混配药剂设置5个不同浓度梯度(蚜虫死亡率在5%~90%的范围内按等比级数设定)。 First, a single agent and each compounding agents set five different concentrations (Aphid mortality in the range of 5% to 90% set by geometric progression). 试虫为采自田间的蚜虫，在实验室挑选均匀一致的蚜虫，放入带有叶片的培养皿内，每皿40头虫子，每处理4个培养皿，重复3次，用potter喷雾塔在50PSI压力下喷雾，每次喷一个皿，每皿喷药液3ml，然后将处理过的培养皿放入12H/12H光照培养箱内培养，72小时检查死虫数，计算死亡率。 Test insects as aphids collected from the field, in the laboratory selected uniform aphid, placed in a petri dish with blades, 40 insects per dish, each dish handle 4, repeated three times, with a potter in a spray tower Under 50PSI pressure spray, spray each a dish, each dish spraying liquid 3ml, then placed in petri dishes treated 12H / 12H illumination incubator, 72 hours to check the number of dead insects, mortality was calculated. 空白对照喷等量清水。 Blank spray the same amount of water. 通过死亡率的机率值和系列浓度的对数值之间的线性回归分析，求出各药剂的LC5tl值，用孙云沛法计算混剂的共毒系数（CTC)，以此来评价供试药剂对试虫的活性。 By mortality probability values ​​and a series of concentrations of the linear regression analysis between the values ​​obtained LC5tl value of each agent, calculated mixture by co-toxicity coefficient (CTC) by 孙云沛 method, in order to evaluate the test agents on trial activity of insects.

[0023] 复配制剂的共毒系数（CTC)彡120表现为增效作用；CTC < 80表现为拮抗作用； 80 < CTC < 120表现为相加作用。 [0023] Mixture of co-toxicity coefficient (CTC) San 120 showed synergism; CTC <80 were antagonistic; 80 <CTC <120 showed an additive effect.

[0024] [0024]

[0025] [0025]

[0026] 室内毒力测定结果表明：环氧虫啉和联苯菊酯、高效氯氟氰菊酯以重量比为I : 10~20 :1混用对苹果黄蚜有较好的毒力，均有显著的增效作用。 [0026] Toxicity test results showed that: Epoxy insect morpholine and bifenthrin, cyhalothrin weight ratio of I: 10 ~ 20: 1 mix of apple aphid better virulence are There are significant synergies.

[0027] 为了防治农业生产上的各类蝽象，发明人以环氧虫啉分别与联苯菊酯、高效氯氟氰菊酯进行了相互复配的增效研宄。 [0027] In order to combat all kinds of bugs like agricultural production, the inventors epoxy insect morpholine respectively bifenthrin, cyhalothrin were mutually synergistic complex research traitor. 试验采用棉花绿盲蝽为测试对象，具体方法为： Test using cotton lucorum test object, specific methods are:

[0028] 绿盲蝽，学名Apolygus lucorum Meyer-DUr，属半翅目盲蝽科。 [0028] lucorum, scientific Apolygus lucorum Meyer-DUr, genus Hemiptera Pentatomidae blindness. 将原药配制成需要的试验药剂。 Original test drug formulated into pharmaceutical needs. 首先将单剂及各混配药剂设置5个不同浓度梯度(在预备试验结果的基础上， 绿盲蝽死亡率在5%~90%的范围内按等比级数设定)。 First, a single agent and each compounding agents set five different concentrations (based on preliminary test results, lucorum mortality rate in the range of 5% to 90% set by geometric progression). 试虫为棉田采集绿盲蝽成虫，在室内豆角上饲养，至下一代若虫发育到3龄供试。 Test for the cotton fields insect collection lucorum adult, feeding on the interior beans, to the next generation of development to third instar nymphs of the test. 将新鲜大小一致的棉花叶片在药液中浸渍30 s后，于室温条件下自然晾干，而后放入塑料盒（直径8 cm，高5 cm)中，接入活泼健康、大小一致的3龄幼虫，15头/盒，用带有小孔的盒盖盖好。 Fresh cotton leaves the same size in the liquid after dipping 30 s, at room temperature to dry naturally, and then placed in a plastic box (diameter 8 cm, height 5 cm), the access active and healthy, the same size of 3 years old larvae, 15 / box, with a small hole covered with a lid. 另设空白对照，重复3次。 Another blank control, repeated three times. 置温度为26~28 °C、相对湿度为70%左右的光照培养箱内饲养观察。 Set a temperature of 26 ~ 28 ° C, relative humidity of about 70 percent of light within the incubator rearing. 48小时检查试虫的死亡情况。 48 hours to check the test insect mortality. 通过死亡率的机率值和系列浓度的对数值之间的线性回归分析，求出各药剂的LC5tl值，用孙云沛法计算混剂的共毒系数（CTC)，以此来评价供试药剂对试虫的活性。 By mortality probability values ​​and a series of concentrations of the linear regression analysis between the values ​​obtained LC5tl value of each agent, calculated mixture by co-toxicity coefficient (CTC) by 孙云沛 method, in order to evaluate the test agents on trial activity of insects.

[0029] 复配制剂的共毒系数（CTC)彡120表现为增效作用；CTC < 80表现为拮抗作用； 80 < CTC < 120表现为相加作用。 [0029] Mixture of co-toxicity coefficient (CTC) San 120 showed synergism; CTC <80 were antagonistic; 80 <CTC <120 showed an additive effect.

[0030] [0030]

[0032] 室内毒力测定结果表明：环氧虫啉和联苯菊酯、高效氯氟氰菊酯以重量比为I : 10~20 :1混用对绿盲蝽有较好的毒力，均有增效作用。 [0032] Toxicity test results showed that: Epoxy insect morpholine and bifenthrin, cyhalothrin weight ratio of I: 10 ~ 20: 1 mix of lucorum better virulence are There are synergies.

[0033] 下面结合实施例对本发明作进一步说明。 [0033] The following combination of embodiments of the present invention will be further described below.

[0034] 制剂实施例1 [0034] Formulation Example 1

[0035] 称取10%环氧虫啉、10%联苯菊酯、3% GY-D06 (聚羧酸盐)、3%木质素磺酸钠、1% TERSPERSE2020、1% K-12 (十二烷基硫酸钠)、1%PVA (分子量2000)、10%聚乙烯吡咯烷酮、高岭土加至100%重量份。 [0035] Weigh 10% epoxy insects morpholine, bifenthrin 10%, 3% GY-D06 (polycarboxylates), 3% sodium lignosulfonate, 1% TERSPERSE2020,1% K-12 (ten sodium lauryl sulfate), 1% PVA (molecular weight 2000), 10% polyvinylpyrrolidone, kaolin added to 100% by weight. 上述原料经常规制取水分散粒剂的方法即混合、超微气流粉碎、混合、造粒步骤制取20%环氧虫啉•联苯菊酯水分散粒剂。 The raw material manufactured by conventional water dispersible granules method that is mixed with ultrafine jet milling, mixing, granulating step preparation of 20% ethylene insect morpholine • bifenthrin water dispersible granules.

[0036] 制剂实施例2 [0036] Formulation Example 2

[0037] 称取60%环氧虫啉、20%高效氯氟氰菊酯、2% TERSPERSE 2700 (聚羧酸盐，美国亨斯迈公司出品)、2%扩散剂NNO (烷基萘磺酸盐甲醛缩合物)、2%拉开粉BX (二丁基萘磺酸钠)、2%K-12、2%硅藻土、1%尿素、高岭土加至100%重量份。 [0037] Weigh 60% epoxy insect morpholine, 20% cyhalothrin, 2% TERSPERSE 2700 (polycarboxylate, Huntsman Corp.), 2% diffusing agent NNO (alkyl naphthalene sulfonic acid Salt formaldehyde condensates), 2% Nekal BX (dibutyl naphthalene sulfonate), 2% K-12,2% diatomaceous earth, 1% urea, kaolin added to 100% by weight. 上述原料经常规制取水分散粒剂的方法即混合、超微气流粉碎、混合、造粒步骤制取80%环氧虫啉·高效氯氟氰菊酯水分散粒剂。 The raw material manufactured by conventional water dispersible granules method that is mixed with ultrafine jet milling, mixing, granulating step preparation of 80% ethylene insect morpholine · cyhalothrin water dispersible granules.

[0038] 制剂实施例3 [0038] Formulation Example 3

[0039] 称取60%环氧虫啉、3%联苯菊醋、6%木质素磺酸1¾ (Ufoxane 3Α)、3%烷基萘磺酸盐甲醛缩合物（Tamol ΝΝ)、1%十二烷基硫酸钠、5%白炭黑、高岭土加足至100%的重量份。 [0039] Weigh 60% epoxy insects morpholine, 3% biphenyl chrysanthemum vinegar, 6% lignosulfonate 1¾ (Ufoxane 3Α), 3% alkylnaphthalene sulfonate formaldehyde condensate (Tamol ΝΝ), 1% ten sodium lauryl sulfate, 5% white carbon, kaolin enough to add 100% by weight. 上述原料经混合、超微气流粉碎、混合工艺步骤制备得63%环氧虫啉•联苯菊酯可湿性粉剂。 The raw material has been mixed ultrafine jet milling, mixing process step preparation was 63% ethylene insect morpholine • bifenthrin wettable powder.

[0040] 制剂实施例4 [0040] Formulation Example 4

[0041] 称取5%环氧虫啉、50%高效氯氟氰菊酯、6%木质素磺酸钙、4%烷基萘磺酸盐甲醛缩合物（Morwet D-425)、l. 5%十二烷基硫酸钠、12%白炭黑、轻钙加足至100%的重量份。 [0041] Weigh 5% of epoxy insect morpholine, 50% cyhalothrin, 6% calcium lignin sulfonate, 4% alkyl naphthalene sulfonate formaldehyde condensate (Morwet D-425), l. 5 % sodium dodecyl sulfate, 12% silica, calcium and light enough to 100% by weight. 上述原料经混合、超微气流粉碎、混合工艺步骤制备得55%环氧虫啉•高效氯氟氰菊酯可湿性粉剂。 The raw material has been mixed ultrafine jet milling, mixing process step preparation was 55% ethylene insect morpholine • cyhalothrin wettable powder.

[0042] 制剂实施例5 [0042] Formulation Example 5

[0043] 称取20% 环氧虫啉、4% 联苯菊酯、3%TERSPERSE 4894、1%TERSPERSE 2500、0· 3% 黄原胶、5%丙二醇、0. 5%苯甲酸、0. 5%有机硅消泡剂(商品名：s-29南京四新应用化学品公司出品)、去离子水加至100%重量份。 [0043] Weigh 20% epoxy insects morpholine, bifenthrin 4%, 3% TERSPERSE 4894,1% TERSPERSE 2500,0 · 3% of xanthan gum, 5% propylene glycol, 0.5% benzoic acid, 0. 5% silicone anti-foaming agent (trade name: s-29 Nanjing four new chemicals should Corp.), deionized water was added to 100% by weight. 上述原料经混合，高速剪切分散30min，用砂磨机砂磨后制得24%环氧虫啉•联苯菊醋悬浮剂。 After mixing the raw material, high-speed shearing dispersion 30min, after sanding with a sanding machine to obtain 24% of ethylene insect morpholine • biphenyl chrysanthemum vinegar suspensions.

[0044] 制剂实施例6 [0044] Formulation Example 6

[0045] 称取10%环氧虫啉、20%高效氯氟氰菊酯、1%TERSPERSE 2020 (美国亨斯迈公司出品）、3%农乳700#、3%农乳1601#、2%TERSPERSE 2500、0. 3%黄原胶、5% 丙二醇、0. 5%苯甲酸、 0. 5%有机硅消泡剂(商品名：s-29南京四新应用化学品公司出品)、去离子水加至100%重量份。 [0045] Weigh 10% epoxy insect morpholine, 20% cyhalothrin, 1% TERSPERSE 2020 (Huntsman Corp.), 700 # 3 percent dairy farming, dairy farming 1601 # 3 percent, 2 percent . TERSPERSE 2500,0 3% xanthan gum, 5% propylene glycol, 05% acid, 0.5% silicone defoamer (trade name: s-29 Nanjing four new chemicals should Corp.), deionized Water to 100% by weight. 上述原料经混合，高速剪切分散30min，用砂磨机砂磨后制得30%环氧虫啉•高效氯氟氰菊酯悬浮剂。 After mixing the raw material, high-speed shearing dispersion 30min, after sanding with a sanding machine to obtain 30% of ethylene insect morpholine • cyhalothrin suspensions.

[0046] 制剂实施例7 [0046] Formulation Example 7

[0047] 称取5%环氧虫啉、25%联苯菊酯、4%农乳500#、2%农乳600#、3%农乳2201#、15% 二甲基甲酰胺、溶剂油S-180加至100%重量份。 [0047] Weigh 5% of epoxy insect morpholine, 25% bifenthrin, 4% Nongru 500 #, 600 # 2% dairy farming, dairy farming 2201 # 3%, 15% dimethyl formamide, solvent oil S-180 to 100% by weight. 上述原料经混合，搅拌溶解完全后制得30% 环氧虫啉•联苯菊醋乳油。 The raw material has been mixed and stirred to dissolve completely to obtain 30% of ethylene after insect morpholine • biphenyl chrysanthemum vinegar EC.

[0048] 制剂实施例8 [0048] Formulation Example 8

[0049] 称取7. 5%环氧虫啉、2. 5%高效氯氟氰菊酯、5%农乳500#、3% TX-KK4%农乳1601#、20%丙酮、二甲苯加至100%重量份。 [0049] Weigh 7.5% ethylene insect morpholine, 2.5% cyhalothrin, 5% Nongru 500 #, 3% TX-KK4% Nongru # 1601, 20% acetone, xylene plus to 100% by weight. 上述原料经混合，搅拌溶解完全后制得10%环氧虫啉•高效氯氟氰菊酯乳油。 The raw material has been mixed and stirred to dissolve completely to obtain 10% of ethylene after insect morpholine • cyhalothrin EC.

[0050] 制剂实施例9 [0050] Formulation Example 9

[0051] 称取2%环氧虫啉、1%联苯菊酯、4%TX-10、5%农乳700#、6%农乳500#、4%农乳1601#、10%环己酮、5%Ν-甲基吡咯烷酮、5%正丁醇，经溶解完全并混合均匀，去离子水加至100%重量份，搅拌后制得3%环氧虫啉•联苯菊酯微乳剂。 [0051] Weigh 2% epoxy insect morpholine, 1% bifenthrin, 4% TX-10,5% Nongru 700 #, 500 # 6 percent dairy farming, dairy farming 1601 # 4%, 10% cyclohexanone ketone, 5% Ν- methylpyrrolidone, 5% n-butanol, dried and dissolved completely mixed, deionized water to 100% by weight, and stirred to obtain 3% ethylene insect morpholine • bifenthrin microemulsion .

[0052] 制剂实施例10 [0052] Formulation Example 10

[0053] 称取5%环氧虫啉、15%高效氯氟氰菊酯、5%农乳700#、3%农乳500#、6%农乳600#、 5%二甲苯、20%环已酮、5%乙醇，经溶解完全并混合均匀，去离子水加至100%重量份，搅拌后制得20%环氧虫啉•高效氯氟氰菊酯微乳剂。 [0053] Weigh 5% of epoxy insect morpholine, cyhalothrin 15%, 5% Nongru 700 #, 500 # 3 percent dairy farming, dairy farming 600 # 6%, 5% xylene, 20% ring cyclohexanone, 5% ethanol, dried and dissolved completely mixed with deionized water to 100% by weight, and stirred to obtain 20% of ethylene insect morpholine • cyhalothrin microemulsion.

[0054] 生物实施例1 :防治棉盲蝽、棉蚜田间试验 1 [0054] Biological Examples: Combat cotton bugs, cotton aphid field trials

[0055] 发明人于2013年7月在陕西省渭南市临渭区做了制剂实例1 (20%环氧虫啉•联苯菊酯水分散粒剂)、制剂实施例8 (10%环氧虫啉•高效氯氟氰菊酯乳油)防治棉盲蝽、棉蚜田间药效试验，试验参照《农药田间药效试验准则HY/T 1464. 3-2007杀虫剂防治棉盲蝽》 和《农药田间药效试验准则（二）GB/T 17980. 75-2004杀虫剂防治棉花蚜虫》。 [0055] The inventors in July 2013 in Weinan City, Shaanxi Province Linwei done Formulation Example 1 (20% ethylene insect morpholine • bifenthrin water dispersible granules), Formulation Example 8 (10% ethylene insect morpholine • cyhalothrin EC) Prevention cotton bugs, cotton aphid field efficacy trials, the test reference "pesticides field efficacy trials HY / T 1464. 3-2007 insecticides against cotton plant bugs" and " Pesticide Guidelines for the field efficacy trials (two) GB / T 17980. 75-2004 Insecticides against cotton aphids. " 试验作物为棉花，防治对象为棉花绿盲蝽、棉蚜。 Test crops are cotton, cotton Control Target lucorum, cotton aphid. 试验棉花田土壤为粘土，肥力中等，各小区的栽培条件、水肥管理均匀一致。 Test cotton field soil is clay, medium fertility, cultivation conditions for each cell, the uniform water and fertilizer management. 两种试验对象均发生较重。 Two test subjects were heavier occur. 7月20日施药，用药液量为60kg/667 m2,采用手动喷雾器均匀喷施，要求各小区棉花叶片正反面、边尖、花蕾均匀着药。 July 20 spraying with liquid amount of 60kg / 667 m2, with manual sprayer evenly spraying, requires each cell cotton leaves positive and negative, sharp edges, bud even the drug. 试验药剂及剂量详见表3。 Test drug and dose are shown in Table 3. 另设空白对照，每处理4次重复，每小区面积50 m2,共40个小区，随机区组排列。 Another blank control, four replicates per treatment, each plot area 50 m2, a total of 40 quarters, randomized block.

[0056] 棉盲蝽调查方法： [0056] Cotton Lygus Survey Methods:

[0057] 施药前调查基数，施药后1、3、7天分别调查残虫数。 [0057] administration base before the survey after survey residual spraying 1,3,7 days, respectively the number of insects. 每小区采用对角线5点取样， 每点连续查10 -15株棉花，并挂牌标记，调查并记录每株棉花上棉盲蝽的成、若虫数量。 5:00 each plot using a diagonal sampling, continuous search cotton plants every 10-15 point, and listed markers, survey and record each plant cotton into cotton bugs, nymphs number. 根据棉盲蝽的成、若虫昼夜节律，宜选择虫体不活跃时进行调查。 According cotton into bugs, nymphs circadian rhythm, an investigation should be chosen when the parasite is not active.

[0058] 棉蚜调查方法： [0058] Aphis Survey Methods:

[0059] 对角线五点取样，每点固定4株，每株固定上、中、下3片叶，共固定调查20株60片叶，调查记录活蚜虫数。 [0059] diagonal five-point sampling, fixed 4 per point per plant fixed, middle and lower leaves were fixed survey 20 60 leaves, the survey recorded live aphids. 药前调查虫口基数，药后1、3、7天各调查1次小区的残存蚜虫数，依据药前虫口基数和药后各天的残虫量计算校正防效。 Prodrug investigation insect population base, 1,3,7 days after drug investigation once each remaining cell number of aphids is calculated based on the residual insect control effect correction amount of each day before and after drug insect population base and medicine.

[0060] 防治棉盲蝽、棉财药效按下面的公式计算： [0060] Prevention of cotton bugs, cotton fiscal efficacy according to the following formula:

[0061] 防治效果（％)=[ 1 -（对照区药前虫数X处理区药后虫数V(对照区药后虫数X 处理区药前虫数）]X 100。 [0061] controlling effect (%) = [l - (former drug control area treated area after X number of insects worm medicine Number V (control area after the worms before drug treatment area X number of insects drug)] X 100.

[0062] 每次调查药效时同时观察各处理区对棉花叶片、花蕾及其它害虫的影响。 [0062] while observing the influence of the treatment zones of Cotton Leaves, buds and other pests in the investigation of the efficacy of each.

[0063] [0063]

[0064] 试验结果表明，20%环氧虫啉·联苯菊酯水分散粒剂3000倍、3500倍、4000倍，10% 环氧虫啉·高效氯氟氰菊酯乳油2000倍、2500倍、3000倍对棉花绿盲蝽、棉蚜有较显著的防治效果，明显优于10%环氧虫啉悬浮剂1000倍、10%联苯菊酯乳油2000倍、2. 5%高效氯氟氰菊酯1000倍的防治效果(表3)。 [0064] The results showed that 20% of ethylene insect morpholine · bifenthrin WDG 3000 times, 3500 times, 4000 times, 10% ethylene insect morpholine · cyhalothrin EC 2000 times, 2500 times 3000 times lucorum cotton, cotton aphid more significant control effect, obviously better than 10% ethylene insect morpholine SC 1000, 10% EC 2000 times bifenthrin, 2.5% cyhalothrin permethrin 1000 times control effects (Table 3).

[0065] 安全性调查，试验期间观察，所有供试药剂对棉花生长安全，无药害现象发生。 [0065] Security Survey, observed during the test, all the tested pesticides on cotton growth and security, no injury occurred. [0066] 试验结果表明，环氧虫啉与联苯菊酯、高效氯氟氰菊酯混配后，明显提高了对棉盲蝽、棉蚜的防治效果，在这两种害虫同时发生时，一次施药达到兼治，降低了用药量，减少了用药次数及用药成本，对棉花生长安全，是防治棉盲蝽、棉蚜的理想药剂。 [0066] The results showed that the epoxy insect morpholine and bifenthrin, cyhalothrin mixed, the significantly increased cotton bugs, cotton aphid control effect, in which the two pests occur simultaneously, a pesticide to rid reached, reducing the dosage, reducing the number of drugs and medication costs for cotton growing security, prevention and treatment of the cotton plant bug, ideal agent Cotton.

[0067] 从以上实施例中可见，第一活性成分环氧虫啉与第二活性成分联苯菊酯或高效氯氟氰菊酯组合复配，可制成水分散粒剂、可湿性粉剂、悬浮剂、乳油或微乳剂剂型，对危害农作物的蚜虫、蝽象有显著的防治效果，用药量少、防治效果高等优点，节约了用药成本和用药量，且对作物安全性良好，因此生产上有广泛的应用前景。 [0067] From the above examples can be seen, the first active ingredient epoxy insect morpholine and a second active ingredient bifenthrin or cyhalothrin complex combinations can be made water dispersible granules, wettable powders, suspensions, creams or microemulsion formulation of crop aphids, bugs like a significant control effect, less medication, control effect advantages, saving the cost of medication and dosage, and safety of the crop is good, so the production on There are a wide range of applications.

[0068] 综上所述，本发明的组合物是采用两种活性成分复配方案，其活性和杀虫效果不是各组分活性的简单叠加，与现有的单一制剂相比，除具有显著的杀虫效果外，而且有显著的增效作用，对作物安全性好，符合农药制剂的安全性要求。 [0068] In summary, the compositions of the present invention is the use of the two active ingredients mixed solution, the simple sum of the activity of each component and the insecticidal effect is not active, compared with the conventional single formulation, in addition to having a significant insecticidal effect, but also has a significant synergistic effect on crop safety, in line with the security requirements of pesticide formulations.